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Operation Specifications of Class A Laminar Flow Car
Operation Specifications of Class A Laminar Flow CarI. Operation Specifications(I) Preparation before Use Environmental Inspection Ensure that the Class A laminar flow car is placed on clean, dry, and flat ground. The surrounding environment should be kept away from excessive movement of people and dust generated by equipment operation. For example, when used in a pharmaceutical workshop, it should be kept away from pulverizing equipment and material transportation channels with large dust production. Check whether the temperature and humidity of the room where the laminar flow car is placed meet the requirements. Generally speaking, the temperature should be controlled at 18-26℃ and the relative humidity at 45%-65%. This is because inappropriate temperature and humidity may affect the airflow stability and equipment performance inside the laminar flow car. Equipment Inspection Before turning on the power of the laminar flow car, check whether the fan, high-efficiency filter, lighting equipment, and other components have obvious signs of damage or looseness. For example, check whether the fan blades are deformed and whether the sealing strips of the high-efficiency filter are intact. Check whether the wind speed adjustment device of the laminar flow car can work properly. Generally, the average wind speed in the working area of the Class A laminar flow car should be maintained at 0.36-0.54m/s. Use an anemometer to measure at different locations in the work area to ensure that the wind speed meets the standard. Check whether the ultraviolet sterilization lamp inside the laminar flow cart can be lit normally, and record its cumulative use time. The ultraviolet sterilization lamp generally needs to be replaced after the cumulative use time reaches the specified time (such as 1000-2000 hours) to ensure the sterilization effect. (II) Operation process Placement of items Place the items that need to be operated in the Class A laminar flow cart (such as medicines, instruments, experimental materials, etc.) slowly and steadily on the workbench. Avoid excessive movements that cause airflow turbulence and affect the laminar flow effect. For example, when placing sterile drugs, you should gently hold up the drug packaging with both hands and put it in steadily from the opening of the laminar flow cart. Ensure that there is an appropriate distance between the items and they cannot be stacked too high to avoid blocking the airflow. Generally speaking, the height of the items should not exceed two-thirds of the height of the interior of the laminar flow cart, and the gap between adjacent items should not be less than 10-15 cm. Operational action specifications The operator should stand in front of the laminar flow vehicle, avoid standing on the side or back to operate, and prevent the particles and microorganisms emitted by the human body from interfering with the clean environment in the laminar flow vehicle. During operation, the arm should be slowly extended into the laminar flow vehicle to avoid rapid movement to cause airflow disturbance. When performing operations such as drug packaging and instrument assembly, the tools used (such as tweezers, scissors, etc.) should be cleaned and disinfected in advance, and handled with care. For example, when using tweezers to pick up drugs, the tweezers should be vertically in and out of the laminar flow vehicle, and the movements should be gentle and accurate. If stirring, mixing, etc. are required in the laminar flow vehicle, appropriate, sterilized tools should be used, and the operation speed should be moderate. For example, when mixing cell culture reagents, sterile glass rods or electric stirrers should be used, and the stirring speed should not be too fast to avoid liquid splashing. Fan and lighting control During operation, ensure that the fan is running continuously and the fan must not be turned off at will. Once the fan stops, the laminar state is destroyed, which will cause external pollutants to enter the laminar flow vehicle. Reasonably adjust the lighting brightness according to operational needs. Avoid too bright or too dark light that affects the operator's vision. At the same time, pay attention to the heat dissipation of the lighting equipment so that it does not have an adverse effect on the internal environment of the laminar flow vehicle. (III) Cleaning after use Item cleaning After the operation is completed, carefully remove the items in the laminar flow vehicle and place them in a pre-prepared clean, and sterile container or area. For used disposable items, such as gloves, straws, etc., they should be immediately placed in medical waste or special garbage collection containers. Reusable items, such as laboratory equipment and tools, should be cleaned and disinfected promptly. For example, metal instruments should be rinsed with clean water first, and then soaked and disinfected with a suitable disinfectant (such as 75% alcohol). Equipment cleaning After turning off the power of the laminar flow vehicle, use a clean, soft damp cloth or a special detergent to wipe the work surface, side walls, etc. inside the laminar flow vehicle. Be careful not to use detergents containing corrosive ingredients to avoid damaging the surface of the equipment. When cleaning the surface dust of the high-efficiency filter, use a vacuum cleaner or a soft brush to gently clean it to avoid excessive force to damage the filter. The inside of the filter is generally maintained and replaced by professionals according to the prescribed cycle. Clean the dust and stains on the outside of the laminar flow vehicle to keep the appearance of the equipment clean and tidy. At the same time, check whether the power cord of the equipment is intact, damaged, or aged. If there is any problem, it should be dealt with in time. |
