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Daily operation of vertical flow work cabinet
Daily operation of vertical flow work cabinetBefore starting: prepare thoroughly and lay the foundationEnvironmental cleaning: First of all, ensure that the area around the workbench is clean and tidy, with no debris accumulation, and no dust. You can wipe the surrounding table and ground with a wet cloth to prevent the external dust from being sucked into the workbench after starting. Visual examination: Approach the vertical flow table and examine its appearance as carefully as a doctor would do a preoperative examination. If the shell is damaged or cracked, observe whether the indicator light and button on the operation panel are intact and whether the display screen is abnormal. If any problem is found, it should be marked in time and reported for repair. Preparation: Prepare tools, utensils, reagents, etc. that will be used in the workbench in advance, and make sure that they have been cleaned or sterilized. For example, glassware should be washed and dried, and metal instruments should be autoclaved at high temperatures to avoid bringing pollution sources to the workbench. Boot: orderly start, activate efficiency Power on: After confirming all preparations are correct, insert the plug into the suitable power socket. Note that the socket must be well grounded to prevent leakage. At this point, a slight electrical noise can be heard inside the device, indicating that it has begun to enter the standby state. Start the fan: Press the fan start button on the operation panel to start the fan. At first, the wind speed may not be stable, don't worry, wait a moment, it will gradually level off. At the same time, pay attention to listening to the fan running sound is normal, there is no abnormal friction or vibration noise, if there is a similar to sharp or dull abnormal sound, you need to stop immediately troubleshooting. Normally, with the fan turned on, the airflow on the workbench begins to flow vertically down or up (depending on the type of equipment) at a uniform speed, creating a clean atmosphere for the operation to begin. Check airflow and pressure: Most advanced vertical flow tables have airflow monitoring and pressure displays. The airflow value on the display screen should be within the standard range specified in the equipment manual, and it is generally better to form a stable uniform, laminar flow. The pressure indication should also be normal, if the pressure is insufficient or too high, it may imply problems such as filter blockage, fan failure, etc., which should be analyzed and handled according to the specific situation. In operation: be cautious, and guard clean. Item placement: Turn on the UV lamp (if necessary) to sterilize the inside of the workbench in advance for some time, turn off the UV lamp, and turn on the lighting. Wash and dry your hands or put on sterile gloves, and gently and slowly put the prepared items into the workbench from the operating window, taking care not to move your arms quickly to avoid disturbing the airflow. Items should be placed in an orderly manner, and try not to block the outlet and intake to ensure smooth circulation of air. For example, when performing cell culture operations, the Petri dishes should be neatly arranged in the center of the operation area, and peripheral auxiliary tools such as pipettes and test tube racks should be placed in a place that does not affect the operation and does not interfere with the airflow. Operation specification: Formal operation, the action should be gentle and precise. Keep the arm in and out of the operation window as level and slow as possible to reduce the introduction of outside air. If it is to carry out aseptic inoculation and other operations, the inoculation tool should be burned on the flame of the alcohol lamp to sterilize before and after use, and pay attention to not allowing the flame to disturb the airflow too much when burning, and avoid burning the hot tool to burn the high-efficiency filter inside the workbench. In the process of adding reagents, the bottle mouth should not face the operating table to prevent the reagent dripping pollution, and the reagent bottle should be closed in time after opening to reduce the pollution of volatile substances to the environment. Shutdown: Finish well, extend life Clean the table: After the operation task is completed, do not rush to shut down. First, carefully remove the items from the workbench, classify the discarded items according to the biosafety or environmental protection requirements, and bring out the reusable tools and utensils for cleaning and sterilization in time. Wipe the table with a special residue-free cleaner and sterile gauze, remove residual reagents, cell debris, and other impurities, and wipe the direction along the direction of the airflow, to avoid the reverse direction of wiping caused by secondary pollution. Turn off the equipment: After confirming that the table is clean and all tools and items are returned, press the fan stop button first. When the fan stops running completely and the wind speed drops to zero, cut off the power supply. The order of this step must not be reversed, otherwise, a sudden power failure may damage the fan and shorten the service life of the equipment. Finally, close the operation window, close the protective curtain (if equipped), and let the vertical flow table "rest" in a clean and safe state, waiting for the next activation. Mastering the daily operation points of the vertical flow table is not only responsible for the quality of work but also the key to extending the life of the equipment and ensuring the smooth progress of each experiment and production task. I hope this guide will help you along the road to clean operation. |
