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Daily operation and maintenance of a clean workbench
Daily operation and maintenance of a clean workbench1. Preparation before operationEnvironmental inspection The clean workbench should be placed in a clean, dry, and well-ventilated indoor environment. Avoid placing it near sources of pollution, such as chemical reagent storage areas and dusty channels. Ensure that the ground level of the installation location is smooth to prevent the workbench from shaking affecting its normal operation. Indoor temperature and humidity also need to be controlled within a suitable range. Generally speaking, the temperature is maintained at 18-26 degrees Celsius, and the relative humidity is ideal at 40%-60%. Extreme temperatures and humidity can affect the filter's performance and efficiency. Equipment inspection Before use, it is necessary to check whether the appearance of the clean workbench is damaged, such as whether the shell is cracked, and whether the operation panel is normal. At the same time, check whether the fan can operate normally, and you can preliminarily judge by listening to the sound of the fan running. Under normal circumstances, the fan runs smoothly, without abnormal noise. Check that the HEPA filter is installed correctly and free from damage. A high-efficiency filter is a key component to ensuring cleanliness. If the filter is found to have obvious damage or poor sealing, it should be replaced or repaired in time; otherwise, it will affect the filtration effect. Second, the correct operation steps Boot preheating After the power is turned on, turn on the fan of the clean workbench. Generally, it is necessary to preheat for 10-15 minutes to let the fan run stably so that the airflow inside the clean workbench reaches a stable state. During the preheating process, you can observe whether the parameters such as wind speed and pressure difference displayed on the operation panel are normal. Item placement and operation Place items and tools that need to be operated on a clean workbench through a transfer window (if available) or by appropriate means into the workbench. Items should be placed orderly to avoid blocking the air outlet or air intake. In the operation process, the operator's action should be gentle and slow, and try to reduce the large movement, so as not to disturb the airflow in the workbench. For example, when performing cell culture operations, tools such as Petri dishes and pipettes should be reasonably placed in the operating area. When operating, in accordance with aseptic operation specifications, such as the use of flame-burning pipette gun head disinfection, while paying attention to the hands do not frequently cross the sterile area, to prevent the introduction of external microorganisms. Shutdown steps after the operation is complete. After the operation is complete, first clean the items in the workbench. The used tools and remaining experimental materials are removed from the workbench and processed accordingly. Then turn off the fan, and finally cut off the power. Three, daily maintenance points Cleaning work Clean the shell of the clean workbench regularly (usually at least once a week). You can wipe the shell with a clean, damp cloth to remove dust and stains. However, be careful to avoid water entering the inside of the workbench and damaging the electrical components. The operating table inside the workbench should be wiped and disinfected with disinfectant after each use. Commonly used disinfectants such as 70%-75% ethanol solution, in the disinfection to ensure that all corners of the table can be disinfected. Filter maintenance The pre-filter needs to be cleaned or replaced regularly. Pre-filter is mainly to filter larger particles of dust and other impurities, generally every 1-3 months to clean once, if found that the pre-filter is seriously clogged, should be cleaned in time. When cleaning, you can rinse with water, and then naturally dry after re-installation. The service life of HEPA filters is generally about 1-3 years, depending on the use environment and frequency. When the resistance of the high-efficiency filter increases to a certain extent (which can be monitored by the pressure difference meter) or the filtration efficiency is significantly reduced, it needs to be replaced in time. Replacing the HEPA filter needs to be operated by a professional in the operating manual to ensure that the installation is correct and the seal is good. Fan maintenance The fan is the power source of the clean workbench. Check the fan belt (if any) regularly (every 3-6 months) for looseness or wear. If the belt is found to be loose, it is necessary to adjust the tension in time; If the belt is seriously worn, it should be replaced in time. At the same time, check whether the motor of the fan is running normally, such as checking whether the temperature of the motor is too high and whether the bearing of the motor has an abnormal sound. If the motor fails, it should be repaired or replaced in time to ensure the normal operation of the fan. 4. Troubleshooting and precautions Common troubleshooting If the wind speed in the clean workbench is found to be too low, first check whether the fan is operating normally. If the fan is normal, it may be caused by a blocked filter. You can first check whether the pre-filter needs to be cleaned, and then consider whether the high-efficiency filter needs to be replaced. When the indicator light on the operation panel is abnormally blinking or alarming, you should refer to the instruction manual of the device to check the corresponding fault code, which may be caused by power failure, sensor failure, or filter failure. Matters needing attention Non-professionals should not disassemble the internal components of the clean workbench at will, so as not to damage the equipment or affect its performance. During the operation, if any peculiar smell or abnormal sound is found, stop the operation immediately and check the cause. At the same time, when maintaining and repairing the equipment, be sure to cut off the power supply first to ensure safety. Moreover, all tools and materials used for cleaning and maintenance should be clean to prevent secondary contamination. |
