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BIBO filter sterilization solution
BIBO filter sterilization solutionFilter sterilization solutionWith the global outbreak of the COVID-19 pandemic, high-level biosafety laboratories and vaccine production workshops have received increasing attention from countries around the world. At present, COVID-19 vaccine production workshops and P3 laboratories are being established in various parts of the country. Due to its particularity, high-level biosafety laboratories generally use negative pressure systems, and the air discharged to the atmosphere must also pass through a double-stage HEPA bag-in-bag-out filter (filter) to protect the outdoor air from pollution. Therefore, the filter is an enrichment area for such microorganisms and needs to be sterilized regularly, especially before replacing the HEPA filter every year. It must be sterilized first and can only be replaced after sterilization is qualified. Difficulties in sterilization of filters 1. At present, there is no better method for sterilization in China. The traditional disinfection and sterilization mode of formaldehyde or ozone is used, but it has two problems: it cannot guarantee the occupational safety and health of personnel and cannot guarantee the sterilization effect of the filtration system. 2. The VHP sterilization method is adopted. Because of the double-stage H13 or higher-level high-efficiency filter, the airflow has a significant pressure drop after passing through the filter, and the double-stage high efficiency has a very obvious interception of VHP. If the VHP particle size is too large (such as dry fog), it will be intercepted by the filter; if condensation occurs during the sterilization process, the filter will be blocked. Therefore, the sterilization solution needs to choose: gas VHP (penetrable and efficient), dry process (no condensation), and large circulating air volume (to ensure penetration) to ensure the sterilization effect and distribution uniformity of VHP. Filter sterilization solution Veco Biotech has specially developed a vaporized hydrogen peroxide sterilizer for filters, mainly for sterilizing multi-stage filters. Its sterilization solution has the advantages of easy modification, no condensation, high penetration, good sterilization effect, repeatable sterilization process, easy verification, and thorough sterilization. Special hydrogen peroxide sterilizer Filter sterilization solution introduction Veco Biotech's special vaporized hydrogen peroxide sterilizer. The entire sterilization process is divided into the following steps: 1. Simple modification of the high-efficiency filter The entire high-efficiency filter system needs to have 2 reserved ports, one as the air inlet of hydrogen peroxide gas (circulation disinfection port A), and one as the return air inlet of hydrogen peroxide gas (circulation disinfection port B). If conditions permit, each section of the cabin has 2 reserved ports The high-efficiency filter has 2 reserved ports, one inlet and one outlet 2. Install VHP vaporized hydrogen peroxide sterilizer V high-efficiency filter sterilization 3. We will place the biological indicator (thermophilic stearobacillus spores, bacterial content: 10^6) and chemical indicator used for effect verification in the first 4 groups of high-efficiency filters, the middle 4 groups of the first and second filters, the 4 groups after the second filter, and 1 group in the hose of the sterilizer loop. Each group contains 1 chemical indicator and 1 biological indicator. After 3 hours of sterilization, it can be found that the chemical indicator in the sterilization loop hose has completely changed color. After degrading hydrogen peroxide, close the filter connection pipe, open the filter hatch, take out the high-efficiency filter, and take out all indicators. Observe the color change results of the chemical indicators, and all chemical indicators have completely changed color. The biological indicators of the experimental group and the positive control are inoculated into a TSB culture respectively. After 7 days, the culture results were observed. There was no microbial growth in the culture medium of all experimental groups and the negative control group (purple), and there was microbial growth in the culture medium of the positive control group (yellow), which can prove that the indicators used for the challenge have passed the challenge and are qualified for sterilization. Veken Bio's B-specific vaporized hydrogen peroxide sterilizer is designed for sterilization and has processed filters of multiple specifications such as 12m³ and 24m³. |
